Full Project – THE PHYTOCHEMICAL CONSTITUENTS OF HEXANOLIC EXTRACT OF OCIMUM GRATISSIMUM (SCENT LEAVES)

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CHAPTER ONE

1.0     Introduction

1.1     Aim of Study

1.2     Literature Review

1.2.1  Phytochemical Screening

1.2.1.1 Flavonoid

1.2.1.2 Saponin

1.2.1.3 Tannin

1.2.1.4 Alkaloids

CHAPTER TWO

2.0     Materials and Methods

2.1     Materials Used

2.2     Reagents

2.3     Identification and Preparation of plant materials

2.4     Phytochemical analysis of hexanolic extract of O. gratissimum

2.4.1  Qualitative determination

2.4.1.1 Test for Alkaloids

2.4.1.2 Test for Flavonoids

2.4.1.3 Test for Saponin

2.4.1.4 Test for Phytosterol

2.4.1.5  Test for Phenol

2.4.1.6 Test for Steroids

2.4.1.1 Test for Tannins

2.4.2 Quantitative determination

2.4.2.1 Alkaloids determination

2.4.2.2 Saponin determination

2.4.2.3 Tannin determination

2.4.2.4 Flavonoid determination

2.4.2.5 Estimation of total Phenolic content

2.4.2.6 Reducing Power

CHAPTER THREE

3.0     Results

3.1     Alkaloid determination

3.2     Saponin determination

3.3     Tannin determination

CHAPTER FOUR

4.0     Discussion and Conclusion

4.1     Discussion

4.2     Conclusion

References

LIST OF TABLES

Table 1 – Showing the qualitative and phytochemical analysis of Ocimum grattissimum                                                                            

Table 2 – Showing the tannic acid absorbance

Table 3 – Showing the quercetin absorbance

Table 4 – Showing the absorbance of reducing power

Table 5 – Showing the determination of total Phenolic content

Table 6 – Showing the phytochemical analysis of hexanolic extract of ocimum gratissimum 

Table 7 – Bioactivity of compounds detected through GC-MS screening of hexane extract of scent leaves

LIST OF FIGURES

Figxure 1 – Graph showing the tannic acid standard curve

Figure 2 – Graph showing the quercetin standard curve

Figure 3 – Graph showing the determination of reducing power

Figure 4 – Graph showing the determination of total phenolic content

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Full Project – THE PHYTOCHEMICAL CONSTITUENTS OF HEXANOLIC EXTRACT OF OCIMUM GRATISSIMUM (SCENT LEAVES)