Full Project – THE RESPONSE OF LIPID PROFILE TO THE ADMINISTRATION OF MEZONEURON BENTHAMIANUM BAILL (JENIFIRAN) ETHANOLIC EXTRACT IN ADULT WISTAR RATS

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ABSTRACT

This research work was carried out to determine the effect of Menzoneuron benthamainum on fasting blood sugar level of adult wistar rats. Twenty wistar rats (both male and female) weighing between 140g to 160g were used for this research project. They were acclimatized for 14 days after which the animals were grouped into four groups of five rats each. Each animal were administered based on their body weight. The dose given to animals in group A was 300mg/kg body weight of Menzoneuron benthamainum, Group B was given 500mg/kg body weight of Menzoneuron benthamainum, Group C was given 700mg/kg body weight of Menzoneuron benthamainum and group D (control group) was given distilled water. The administration lasted for thirteen days. Blood samples were collected from the tail of the animals and the Lipid profile level for each of the animals was tested.

TABLE OF CONTENTS

Contents

Title page                                                                                        

Certification                                                                                               

Dedication                                                                                     

Acknowledgements                                                                                

Table of contents                                                                        

List of Figures                                                                                

List of Table                                                                                           

Abstract                                                                                            

CHAPTER ONE

1.0     Introduction

1.1     Medicinal Plants

1.2     Aim of Study

CHAPTER TWO

2.0     Literature review

2.1     Medicinal Plants and their uses

2.1.1 Mezoneuron benthamianum baill                                      

2.2     Lipid Profile

2.2.1  Effect of medicinal plants on Lipid profile

CHAPTER THREE

3.0     Materials and Methods

3.1     Apparatus and Equipments

3.2     Chemical Reagents

3.3     Collection and extraction of plant materials

3.3.1 Mezoneuron benthamianum baill

3.4        Practical history and animal management

3.5        Drug administration

3.6        Sacrificing of Rats

3.7        Biochemical Analysis

3.7.1      Triglycerides

3.7.1.1 Principle

3.7.1.2 Reagent composition

3.7.1.3 Preparation of reagents

3.7.1.4 Procedure

3.7.2  HDL- Cholesterol

3.7.2.1 Principle

3.7.2.2 Preparation of reagents

3.7.2.3 Procedure

3.8     Statistical Analysis

CHAPTER FOUR

4.0     Results

CHAPTER FIVE

5.0     Discussion, Conclusion and Recommendation

5.1     Discussion

5.2     Conclusion

5.3     Recommendation

References

LIST OF FIGURES

Figure 1 –Mezoneuron benthamianum baill                                            

Figure 2 – Bar chart showing the level of cholesterol in adult wistar rats

Figure 3 – Bar chart showing the level of HDL in adult wistar rats

Figure 4 – Bar chart showing the level of LDL in adult wistar rats

Figure 5 – Bar chart showing the level of triglycerides in adult wistar rats

LIST OF TABLE

Table 1 – Groups and treatment administered

Table 2- Procedure triglyceride determination

Table 3 – Procedure for HDL determination

Table 4- Cholesterol CHOD-PAP assay

Table 5- Showing the level of cholesterol, HDL, LDL and

triglycerides in adult wistar rats.

 

 

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Full Project – THE RESPONSE OF LIPID PROFILE TO THE ADMINISTRATION OF MEZONEURON BENTHAMIANUM BAILL (JENIFIRAN) ETHANOLIC EXTRACT IN ADULT WISTAR RATS